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primer transcript ii reverse transcriptase  (TaKaRa)


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    Structured Review

    TaKaRa primer transcript ii reverse transcriptase
    Primer Transcript Ii Reverse Transcriptase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 725 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primer+transcript+ii+reverse+transcriptase/PrimeScript+II+Reverse+Transcriptase/bio_rxiv__64898__2025__12__12__693956-154-37-42
    Average 96 stars, based on 725 article reviews
    primer transcript ii reverse transcriptase - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Lysis:

    Article Title: A long-acting recombinant FSH supports high-quality mouse follicle development and oocyte maturation in vitro by coordinating somatic and germ cell transcriptomes.
    Article Snippet: Strategies to maximize individual fertility chances are constant requirements of ART.. In vitro folliculogenesis may represent a valid option to create a large source of immature ovarian follicles in ART.. Efforts are being made to set up mammalian follicle culture protocols with suitable FSH stimuli.

    Article Title: N-acetyltransferase 10-mediated mRNA N4-acetylation is Essential for the Translational Regulation During Oocyte Meiotic Maturation in Mice
    Article Snippet: .. Five oocytes or embryos were collected and lysed in 2 μL of lysis buffer (0.2% Triton X-100 and 4 IU RNase inhibitor), and cDNA synthesis was followed by retrotranscribing reverse transcription with primer transcript II reverse transcriptase (Takara) according to the manufacturer’s protocol. .. Quantitative RT-PCR analysis was performed using Power SYBR Green PCR Master Mix (Applied Biosystems, Life Technologies) and an Applied Biosystems ABI 7500 Real-Time PCR system using the primers listed in the supplementary material.

    Article Title: Dynamic mRNA degradome analyses indicate a role of histone H3K4 trimethylation in association with meiosis-coupled mRNA decay in oocyte aging
    Article Snippet: Mean intensity of the HPG signal was measured across the middle of each oocyte and quantified using ImageJ software . .. Oocytes or embryos were collected and lysed in 2 μl of lysis buffer (0.2% Triton X-100 and 4 IU RNase inhibitor), followed by reverse transcription with primer transcript II reverse transcriptase (Takara), according to the manufacturer’s instructions. .. Real-time RT-PCR analysis was performed using the LightCycler 480 SYBR Green I Master (Roche) and a Roche 480 Real-Time PCR System.

    Article Title: RAFTER: a releasing factor tethered RNA editing system for quantifying mRNA translation
    Article Snippet: Library quality was assessed using a PerkinElmer LabChip GX Touch system, and sequencing was performed on the Illumina NovaSeq 6000 platform in PE150 mode. .. For each sample replicate, five oocytes were collected and lysed in 2 μL of lysis buffer (0.2% Triton X-100 and 40 U/mL Recombinant RNase Inhibitor (Takara, 2313A)), and cDNA synthesis was followed by retrotranscribing reverse transcription with primer transcript II reverse transcriptase (Takara, 2690A) according to the manufacturer’s protocol. .. RT-qPCR analysis was performed using Power SYBR Green PCR Master Mix (Vazyme, Q712-02) and an Applied Biosystems QuantStudio 5 Real-Time PCR system using the primers listed in Supplementary Table 3.

    Reverse Transcription:

    Article Title: A long-acting recombinant FSH supports high-quality mouse follicle development and oocyte maturation in vitro by coordinating somatic and germ cell transcriptomes.
    Article Snippet: Strategies to maximize individual fertility chances are constant requirements of ART.. In vitro folliculogenesis may represent a valid option to create a large source of immature ovarian follicles in ART.. Efforts are being made to set up mammalian follicle culture protocols with suitable FSH stimuli.

    Article Title: N-acetyltransferase 10-mediated mRNA N4-acetylation is Essential for the Translational Regulation During Oocyte Meiotic Maturation in Mice
    Article Snippet: .. Five oocytes or embryos were collected and lysed in 2 μL of lysis buffer (0.2% Triton X-100 and 4 IU RNase inhibitor), and cDNA synthesis was followed by retrotranscribing reverse transcription with primer transcript II reverse transcriptase (Takara) according to the manufacturer’s protocol. .. Quantitative RT-PCR analysis was performed using Power SYBR Green PCR Master Mix (Applied Biosystems, Life Technologies) and an Applied Biosystems ABI 7500 Real-Time PCR system using the primers listed in the supplementary material.

    Article Title: Dynamic mRNA degradome analyses indicate a role of histone H3K4 trimethylation in association with meiosis-coupled mRNA decay in oocyte aging
    Article Snippet: Mean intensity of the HPG signal was measured across the middle of each oocyte and quantified using ImageJ software . .. Oocytes or embryos were collected and lysed in 2 μl of lysis buffer (0.2% Triton X-100 and 4 IU RNase inhibitor), followed by reverse transcription with primer transcript II reverse transcriptase (Takara), according to the manufacturer’s instructions. .. Real-time RT-PCR analysis was performed using the LightCycler 480 SYBR Green I Master (Roche) and a Roche 480 Real-Time PCR System.


    Article Title: RAFTER: a releasing factor tethered RNA editing system for quantifying mRNA translation
    Article Snippet: Library quality was assessed using a PerkinElmer LabChip GX Touch system, and sequencing was performed on the Illumina NovaSeq 6000 platform in PE150 mode. .. For each sample replicate, five oocytes were collected and lysed in 2 μL of lysis buffer (0.2% Triton X-100 and 40 U/mL Recombinant RNase Inhibitor (Takara, 2313A)), and cDNA synthesis was followed by retrotranscribing reverse transcription with primer transcript II reverse transcriptase (Takara, 2690A) according to the manufacturer’s protocol. .. RT-qPCR analysis was performed using Power SYBR Green PCR Master Mix (Vazyme, Q712-02) and an Applied Biosystems QuantStudio 5 Real-Time PCR system using the primers listed in Supplementary Table 3.

    cDNA Synthesis:

    Article Title: N-acetyltransferase 10-mediated mRNA N4-acetylation is Essential for the Translational Regulation During Oocyte Meiotic Maturation in Mice
    Article Snippet: .. Five oocytes or embryos were collected and lysed in 2 μL of lysis buffer (0.2% Triton X-100 and 4 IU RNase inhibitor), and cDNA synthesis was followed by retrotranscribing reverse transcription with primer transcript II reverse transcriptase (Takara) according to the manufacturer’s protocol. .. Quantitative RT-PCR analysis was performed using Power SYBR Green PCR Master Mix (Applied Biosystems, Life Technologies) and an Applied Biosystems ABI 7500 Real-Time PCR system using the primers listed in the supplementary material.

    Article Title: RAFTER: a releasing factor tethered RNA editing system for quantifying mRNA translation
    Article Snippet: Library quality was assessed using a PerkinElmer LabChip GX Touch system, and sequencing was performed on the Illumina NovaSeq 6000 platform in PE150 mode. .. For each sample replicate, five oocytes were collected and lysed in 2 μL of lysis buffer (0.2% Triton X-100 and 40 U/mL Recombinant RNase Inhibitor (Takara, 2313A)), and cDNA synthesis was followed by retrotranscribing reverse transcription with primer transcript II reverse transcriptase (Takara, 2690A) according to the manufacturer’s protocol. .. RT-qPCR analysis was performed using Power SYBR Green PCR Master Mix (Vazyme, Q712-02) and an Applied Biosystems QuantStudio 5 Real-Time PCR system using the primers listed in Supplementary Table 3.

    Recombinant:

    Article Title: RAFTER: a releasing factor tethered RNA editing system for quantifying mRNA translation
    Article Snippet: Library quality was assessed using a PerkinElmer LabChip GX Touch system, and sequencing was performed on the Illumina NovaSeq 6000 platform in PE150 mode. .. For each sample replicate, five oocytes were collected and lysed in 2 μL of lysis buffer (0.2% Triton X-100 and 40 U/mL Recombinant RNase Inhibitor (Takara, 2313A)), and cDNA synthesis was followed by retrotranscribing reverse transcription with primer transcript II reverse transcriptase (Takara, 2690A) according to the manufacturer’s protocol. .. RT-qPCR analysis was performed using Power SYBR Green PCR Master Mix (Vazyme, Q712-02) and an Applied Biosystems QuantStudio 5 Real-Time PCR system using the primers listed in Supplementary Table 3.



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